This review focuses on the deamidation of asparagine in monoclonal antibodies, one of the well-known degradation pathways.The importance of close monitoring of deamidation from early developability assessment to in vivo studies bosch 4100 table saw motor replacement is discussed consequentially.The application of chromatographic and electrophoretic instruments for the separation of deamidated variants is described.Current mass five finger death punch a decade of destruction spectrometric approaches for characterization of deamidated antibody variants and localization of modification sites are discussed accordingly.
The review also covers the challenges in bioanalysis of deamidated forms in complex biological matrices during in vivo studies.